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 The Next Step Forward in Silica Engineering
The new range of Optimal® HPLC columns from Capital Analytical respresents the next step forward in porous silica engineering beyond the so-called new generation high purity materials.
The current new generation materials are based on an alternative synthesis route which yields a high purity product with excellent inertness characteristics.
Optimal is more than just another new generation HPLC packing material. Optimal® is manufactured using an entirely new process based on well established, tried and tested silica production techniques coupled with new refining technologies which ultimately leads to a superior material combining the advantages of both the new and traditional approaches to silica manufacturing.
Specifications
The fact that high purity Optimal is manufactured using well established silica engineering techniques means that a wide range of specifications are possible. As a result, we can supply Optimal in pore sizes ranging from 60Å to 1000Å with the most popular being 120Å. Moreover, Optimal is also available in particle sizes ranging from 3µm up to preparative grade sizes.
Shape: Spherical
Phases Available: ODS-H, ODS-L, C8 and Silica
Particle Size: 3µm, 5µm and larger
Mean Pore Diameter: 120Å (300, 500 and 1000Å also available)
Carbon Loading: ODS-H (18%), ODS-L (15%)
Specific Surface Area: 320m2g-1. (120Å material)
 Optimal® ODS-H
Optimal® ODS-H is the most generally applicable of the Optimal® phases and can be used for both specialist applications involving acid and basic compounds or mixtures of both. Optimal® ODS-H is also particularly well suited to general purpose method development.
The absence of active silanols in Optimal is illustrated by this standard test mixture which includes both pyridine and phenol. The lack of peak tailing clearly demonstrates the absence of metallic impurities.
Column: Optimal® ODS-H 5µm
Dimensions: 15cm x 4.6mm
Mobile Phase: 40:60 Acetonitrile:Water
Temperature: 40°C
Flow Rate: 1ml/min
Detection: UV at 254nm
1. Pyridine, 2. Phenol
 Neutral silanol groups can still occasionally cause problems with certain classes of compound. Theobromine, theophylline and caffeine are particularly prone to this effect. In Optimal this effect is elimated by full end capping.
Column: Optimal® ODS-H 5µm
Dimensions: 15cm x 4.6mm
Mobile Phase: 25:75 Methanol:Water
Temperature: 30°C
Flow Rate: 1ml/min
Detection: UV at 254nm
1. Theobromine, 2. Theophylline
3. Caffeine, 4. Phenol
Many compounds such as polyphenols interact irreversibly with the active sites on silica based reversed phase materials. In the case of Optimal ODS-H this problem does not occur, which makes it possible to analyse problematic compounds such as in the example below.
 Column: Optimal® ODS-H 5µm
Dimensions: 15cm x 4.6mm
Mobile Phase: THF:Water:Formic Acid (35:60:5)
Temperature: 40°C
Flow Rate: 0.65Sl/min
Detection: UV at 254nm
Optimal® ODS-L
Optimal® ODS-L has a slightly lower carbon loading (15%) than the ODS-H and is useful for the analysis of a wide range of hydrophilic compounds which are normally not retained by other C18 based packing material.
One particular advantage is that even pure water can be used as the eluent without the need for any organic modifier. Highly polar samples only soluble in water may be separated by this packing material, e.g. sugars and other saccharides (see example below). Optimal ODS-L is also as useful for analyzing hydrophobic samples which are currently chromatographed on competitor C8 materials. Like ODS-H, this packing material is also fully end-capped.
OptimalODS-L is also based on the same high purity silica as the Optimal ODS-H material which ensures the high degree of inertness and lack of peak tailing.
Column: Optimal® ODS-L 5µm
Dimensions: 15cm x 4.6mm
Mobile Phase: 100% Water (HPLC Grade)
Temperature: 40°C
Flow Rate: 1ml/min
Detection: Refractive Index
1. Sucrose
2. Kestose
3. Fructotriose
4. Fructotetraose
Optimal Ordering Information
Catalogue numbers refer to 5µm materials 4.6mm column i.d. only. For 2.1mm or 3.2mm diameter columns please add D2 and D3 respectivley to the standard 4.6mm i.d. columns. Prices for 2.1mm and 3.2mm i.d. columns are as for 4.6mm i.d.
Length |
Phase |
Cat. No. |
Price (£) |
|
2cm (Guard) |
Silica |
5SA102 |
|
5cm |
Silica |
5SA105 |
|
10cm |
Silica |
5SA110 |
|
15cm |
Silica |
5SA115 |
|
20cm |
Silica |
5SA120 |
|
25cm |
Silica |
5SA125 |
|
2cm (Guard) |
ODS-H |
5SD102 |
|
5cm |
ODS-H |
5SD105 |
|
10cm |
ODS-H |
5SD110 |
|
15cm |
ODS-H |
5SD115 |
|
20cm |
ODS-H |
5SD120 |
|
25cm |
ODS-H |
5SD125 |
|
2cm (Guard) |
ODS-L |
5SF102 |
|
5cm |
ODS-L |
5SF105 |
|
10cm |
ODS-L |
5SF110 |
|
15cm |
ODS-L |
5SF115 |
|
20cm |
ODS-L |
5SF120 |
|
25cm |
ODS-L |
5SF125 |
|
2cm (Guard) |
Octyl |
5SG102 |
|
5cm |
Octyl |
5SG105 |
|
10cm |
Octyl |
5SG110 |
|
15cm |
Octyl |
5SG115 |
|
20cm |
Octyl |
5SG120 |
|
25cm |
Octyl |
5SG125 |
|
2cm (Guard) |
ODS-H (30) |
5SK102 |
|
5cm |
ODS-H (30) |
5SK105 |
|
10cm |
ODS-H (30) |
5SK110 |
|
15cm |
ODS-H (30) |
5SK115 |
|
20cm |
ODS-H (30) |
5SK120 |
|
25cm |
ODS-H (30) |
5SK125 |
|
2cm (Guard) |
ODS-L (300Å) |
5SL102 |
|
5cm |
ODS-L (300Å) |
5SL105 |
|
10cm |
ODS-L (300Å) |
5SL110 |
|
15cm |
ODS-L (300Å) |
5SL115 |
|
20cm |
ODS-L (300Å) |
5SL120 |
|
25cm |
ODS-L (300Å) |
5SL125 |
|
|
|
|
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