| |
NaAMPS Coated Capillaries (Negatively Charged pH Independent)
In collaboration with Chemical Solutions in the USA, Electro-Kinetic Technologies have introduced a unique anionic capillary coating based on a stable polymer incorporating sulphonic acid functional groups (abbreviated as NaAMPS) and a corresponding positive charged capillary which is particulary suited to the separation of basic proteins. The NaAMPS for is available in a range of capillaries with different, but pH independent EOF rates. Zero flow capillaries are also available.
NaAMPS capillaries are manufactured under the strictest GMP/GLP conditions in order to ensure reliability and excellent batch to batch reproducability. In contrast to most other coating materials, NaAMPS coated capillaries are extremely stable and have a considerably long lifetime.
 This polymeric coating with surface sulphonic acid groups (NaAMPS coating) has, unlike bare silica, a clearly defined pKa value. The surface is therefore fully ionised over a wide pH range. The leads to a reasonably constant EOF over the same pH range.
The figure on the right shows the comparative EOF rates on bare silica and an EKT capillary with NaAMPS coating.
Effect of pH on EOF for Bare Silica Compared to NaAMPS Coated Silica
When developing methods for bare silica CE by varying the buffer pH, it can become confusing as changes in pH alter both the analtye electrophoretic mobility and the EOF.
The use of NaAMPS coated capillaries allows the user to easily assess the effect the change of pH has on the selectivity for their samples without the inconvenience of EOF changes.
NaAMPS capillaries are available from EKT with 3 different surface charge densities which enable the user to choose which degree of electro-osmotic flow is required.
NaAMPS Capillary Types
High Flow - Where a high electro-osmotic flow is required over a large pH range.
Medium Flow - Medium density surface charge, produces a moderate electro-osmotic flow
Low Flow - Generates a low EOF and is particularly useful for isoelectric focussing avoiding the need for complex and costly additives.
 Advantages of NaAMPS
Decreased Sample - Wall Interactions
pH Independent Electro-Osmotic Flow
Adjustable Electro-Osmotic Flow
Reproducable Manufacturing Process
Good Stability and Lifetime
Acidic Compounds on Bare Silica
Analytes
1. Methyl Phenol (marker),
2. 2-naphthalenesulphonic Acid,
3. Benzenesulphonic Acid
At pH 8.7 the EOF carries the analytes towards the detector despite their own motion in the opposing direction. At pH 4.0 the EOF is no longer strong enough and the acidic sample now migrate in the opposite direction.
 Acidic Compounds on NaAMPS Coated Silica
The use of NaAMPS coated capillaries shows that the separation is very stable to changes in pH which ensures greater method ruggedness.
The use of NaAMPS coated capillaries has the following implications for CE and MECC method development
Improves separation efficiency for biomolecules
improves use pH to adjust selectivity without affecting EOF
ideal for chiral separations
operate MECC over a wide pH range
Ordering Information
The open format capillaries are supplied precut to the required size with the UV window correctly positioned to fit the specific instrument manufacturer’s cartridge system. The capillary ends are expertly cut to ensure precise sampling.
Open Format Please specify both effective length and total length when ordering
Description |
Cat. No. |
Price (£) |
|
0-35cm Coated Capillary (Low Flow) 75µm |
EKE1125 |
|
0-35cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1125-3 |
|
35-65cm Coated Capillary (Low Flow) 75µm |
EKE1150 |
|
35-65cm Coated Capillary (Low Flow) 7m (Pack of 3) |
EKE1150-3 |
|
70-100cm Coated Capillary (Low Flow) 75µm |
EKE1175 |
|
70-100cm Coated Capillary (Low Flow) 7m (Pack of 3) |
EKE1175-3 |
|
0-35cm Coated Capillary (Low Flow) 50µm |
EKF1125 |
|
0-35cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1125-3 |
|
35-65cm Coated Capillary (Low Flow) 50µm |
EKF1150 |
|
35-65cm Coated Capillary (Low Flow) 5m (Pack of 3) |
EKF1150-3 |
|
70-100cm Coated Capillary (Low Flow) 50µm |
EKF1175 |
|
70-100cm Coated Capillary (Low Flow) 5m (Pack of 3) |
EKF1175-3 |
|
Low Flow NaAMPS Coated Capillaries – Cartridge Refit Service
Beckman format Total length is nominal length plus 7cm after detection window
Description |
Cat. No. |
Price (£) |
|
Beckman 20cm Coated Capillary (Low Flow) 75µm |
EKE1225 |
|
Beckman – 20cm Coated Capillary (Low Flow) 7m (Pack of 3) |
EKE1225-3 |
|
Beckman – 50cm Coated Capillary (Low Flow) 7m |
EKE1250 |
|
Beckman 50cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1250-3 |
|
Beckman 70cm Coated Capillary (Low Flow) 75µm |
EKE1275 |
|
Beckman 70cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1275-3 |
|
Beckman 25cm Coated Capillary (Low Flow) 50µm |
EKF1225 |
|
Beckman – 25cm Coated Capillary (Low Flow) 5m (Pack of 3) |
EKF1225-3 |
|
Beckman – 50cm Coated Capillary (Low Flow) 5m |
EKF1250 |
|
Beckman 50cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1250-3 |
|
Beckman 70cm Coated Capillary (Low Flow) 50µm |
EKF1275 |
|
Beckman 70cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1275-3 |
|
HP Format capillary fitted to Hewlett-Packard Cartridge
Description |
Cat. No. |
Price (£) |
|
HP – 25cm Coated Capillary (Low Flow) 7m |
EKE1325 |
|
HP – 25cm Coated Capillary (Low Flow) 7m (Pack of 3) |
EKE1325-3 |
|
HP 50cm Coated Capillary (Low Flow) 75µm |
EKE1350 |
|
HP 50cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1350-3 |
|
HP – 75cm Coated Capillary (Low Flow) 7m |
EKE1375 |
|
HP 75cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1375-3 |
|
HP – 25cm Coated Capillary (Low Flow) 5m |
EKF1325 |
|
HP – 25cm Coated Capillary (Low Flow) 5m (Pack of 3) |
EKF1325-3 |
|
HP 50cm Coated Capillary (Low Flow) 50µm |
EKF1350 |
|
HP 50cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1350-3 |
|
HP – 25cm Coated Capillary (Low Flow) 5m |
EKF1375 |
|
HP 25cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1375-3 |
|
TSP Format Capillary Fitted to TSP Cartridge
Description |
Cat. No. |
Price (£) |
|
TSP 25cm Coated Capillary (Low Flow) 75µm |
EKE1425 |
|
TSP – 25cm Coated Capillary (Low Flow) 7m (Pack of 3) |
EKE1425-3 |
|
TSP – 50cm Coated Capillary (Low Flow) 7m |
EKE1450 |
|
TSP 50cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1450-3 |
|
TSP 75cm Coated Capillary (Low Flow) 75µm |
EKE1475 |
|
TSP 75cm Coated Capillary (Low Flow) 75µm (Pack of 3) |
EKE1475-3 |
|
TSP 25cm Coated Capillary (Low Flow) 50µm |
EKF1425 |
|
TSP – 25cm Coated Capillary (Low Flow) 5m (Pack of 3) |
EKF1425-3 |
|
TSP – 50cm Coated Capillary (Low Flow) 5m |
EKF1450 |
|
TSP 50cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1450-3 |
|
TSP 75cm Coated Capillary (Low Flow) 50µm |
EKF1475 |
|
TSP 75cm Coated Capillary (Low Flow) 50µm (Pack of 3) |
EKF1475-3 |
|
High Flow NaAMPS Coated Capillaries
The open format capillaries are supplied precut to the required size with the UV window correctly positioned to fit the specific instrument manufacture’s cartridge system. The capillary ends are expertly cut to ensure precise sampling.
High Flow NaAMPS Open Format Please specify both effective length and total length when ordering
Description |
Cat. No. |
Price (£) |
|
0-35cm Coated Capillary (High Flow) 7m |
EKI1125 |
|
0-35cm Coated Capillary (High Flow) 75µm (Pack of 3) |
EKI1125-3 |
|
35-65cm Coated Capillary (High Flow) 7m |
EKI1150 |
|
35-65cm Coated Capillary (High Flow) 7m (Pack of 3) |
EKI1150-3 |
|
70-100cm Coated Capillary (High Flow) 7m |
EKI1175 |
|
70-100cm Coated Capillary (High Flow) 7m (Pack of 3) |
EKI1175-3 |
|
0-35cm Coated Capillary (High Flow) 5m |
EKJ1125 |
|
0-35cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1125-3 |
|
35-65cm Coated Capillary (High Flow) 5m |
EKJ1150 |
|
35-65cm Coated Capillary (High Flow) 5m (Pack of 3) |
EKJ1150-3 |
|
70-100cm Coated Capillary (High Flow) 5m |
EKJ1175 |
|
70-100cm Coated Capillary (High Flow) 5m (Pack of 3) |
EKJ1175-3 |
|
High Flow NaAMPS Coated Capillaries – Cartridge Refit Service
Beckman format Total length is nominal length plus 7cm after detection window
Description |
Cat. No. |
Price (£) |
|
Beckman–20cm Coated Capillary(High Flow) 75µm |
EKI1225 |
|
Beckma20cm Coated Capillary(High Flow) 7m (Pack of 3) |
EKI1225-3 |
|
Beckma50cm Coated Capillary(High Flow) 7m |
EKI1250 |
|
Beckman–50cm Coated Capillary(High Flow) 75µm (Pack of 3) |
EKI1250-3 |
|
Beckman–70cm Coated Capillary (High Flow) 7m |
EKI1275 |
|
Beckma70cm Coated Capillary (High Flow) 75µm (Pack of 3) |
EKI1275-3 |
|
Beckman–25cm Coated Capillary (High Flow) 5m |
EKJ1225 |
|
Beckman–25cm Coated Capillary (High Flow) 5m (Pack of 3) |
EKJ1225-3 |
|
Beckma50cm Coated Capillary (High Flow) 50µm |
EKJ1250 |
|
Beckma50cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1250-3 |
|
Beckman–70cm Coated Capillary (High Flow) 5m |
EKJ1275 |
|
Beckma70cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1275-3 |
|
HP Format capillary fitted to Hewlett-Packard Cartridge
Description |
Cat. No. |
Price (£) |
|
HP – 25cm Coated Capillary (High Flow) 75µm |
EKI1325 |
|
HP 25cm Coated Capillary (High Flow) 7m (Pack of 3) |
EKI1325-3 |
|
HP 50cm Coated Capillary (High Flow) 7m |
EKI1350 |
|
HP – 50cm Coated Capillary (High Flow) 75µm (Pack of 3) |
EKI1350-3 |
|
HP – 75cm Coated Capillary (High Flow) 75µm |
EKI1375 |
|
HP – 75cm Coated Capillary (High Flow) 75µm (Pack of 3) |
EKI1375-3 |
|
HP – 25cm Coated Capillary (High Flow) 50µm |
EKJ1325 |
|
HP 25cm Coated Capillary (High Flow) 5m (Pack of 3) |
EKJ1325-3 |
|
HP 50cm Coated Capillary (High Flow) 5m |
EKJ1350 |
|
HP – 50cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1350-3 |
|
HP – 25cm Coated Capillary (High Flow) 50µm |
EKJ1375 |
|
HP – 25cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1375-3 |
|
TSP Format Capillary Fitted to TSP Cartridge
Description |
Cat. No. |
Price (£) |
|
TSP 25cm Coated Capillary (High Flow) 7m |
EKI1425 |
|
TSP 25cm Coated Capillary (High Flow) 7m (Pack of 3) |
EKI1425-3 |
|
TSP – 50cm Coated Capillary (High Flow) 75µm |
EKI1450 |
|
TSP – 50cm Coated Capillary (High Flow) 75µm (Pack of 3) |
EKI1450-3 |
|
TSP 75cm Coated Capillary (High Flow) 7m |
EKI1475 |
|
TSP – 75cm Coated Capillary (High Flow) 75µm (Pack of 3) |
EKI1475-3 |
|
TSP 25cm Coated Capillary (High Flow) 5m |
EKJ1425 |
|
TSP 25cm Coated Capillary (High Flow) 5m (Pack of 3) |
EKJ1425-3 |
|
TSP – 50cm Coated Capillary (High Flow) 50µm |
EKJ1450 |
|
TSP – 50cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1450-3 |
|
TSP 75cm Coated Capillary (High Flow) 5m |
EKJ1475 |
|
TSP – 75cm Coated Capillary (High Flow) 50µm (Pack of 3) |
EKJ1475-3 |
|
Medium Flow NaAMPS Coated Capillaries
The open format capillaries are supplied precut to the required size with the UV window correctly positioned to fit the specific instrument manufacture’s cartridge system. The capillary ends are expertly cut to ensure precise sampling.
Medium Flow NaAMPS Open Format Please specify both effective length and total length when ordering
Description |
Cat. No. |
Price (£) |
|
0-35cm Coated Capillary (Medium Flow) 7m |
EKG1125 |
|
0-35cm Coated Capillary (Medium Flow) 75µm (Pack of 3) |
EKG1125-3 |
|
35-65cm Coated Capillary (Medium Flow) 7m |
EKG1150 |
|
35-65cm Coated Capillary (Medium Flow) 7m (Pack of 3) |
EKG1150-3 |
|
70-100cm Coated Capillary (Medium Flow) 7m |
EKG1175 |
|
70-100cm Coated Capillary (Medium Flow) 7m (Pack of 3) |
EKG1175-3 |
|
0-35cm Coated Capillary (Medium Flow) 5m |
EKH1125 |
|
0-35cm Coated Capillary (Medium Flow) 50µm (Pack of 3) |
EKH1125-3 |
|
35-65cm Coated Capillary (Medium Flow) 5m |
EKH1150 |
|
35-65cm Coated Capillary (Medium Flow) 5m (Pack of 3) |
EKH1150-3 |
|
70-100cm Coated Capillary (Medium Flow) 5m |
EKH1175 |
|
70-100cm Coated Capillary (Medium Flow) 5m (Pack of 3) |
EKH1175-3 |
|
Medium Flow NaAMPS Coated Capillaries – Cartridge Refit Service
Beckman format Total length is nominal length plus 7cm after detection window
Description |
Cat. No. |
Price (£) |
|
Beckman-20cm Coated Capillary(Medium Flow)7m |
EKG1225 |
|
Beckman-20cm Coated Capillary(Medium Flow)7m (Pack of 3) |
EKG1225-3 |
|
Beckma50cm Coated Capillary(Medium Flow) 7m |
EKG1250 |
|
Beckman–50cm Coated Capillary(Medium Flow) 75µm (Pack of 3) |
EKG1250-3 |
|
Beckman-70cm Coated Capillary(Medium flow) 75µm |
EKG1275 |
|
Beckman-70cm Coated Capillary(Medium flow) 75µm (Pack of 3) |
EKG1275-3 |
|
Beckman-25cm Coated CapillaryMedium Flow) 5m |
EKG1225 |
|
Beckman-25cm Coated CapillaryMedium Flow) 5m (Pack of 3) |
EKG1225-3 |
|
Beckma50cm Coated Capillary(Medium Flow) 5m |
EKG1250 |
|
Beckman–50cm Coated Capillary(Medium Flow) 50µm (Pack of 3) |
EKG1250-3 |
|
Beckman–70cm Coated Capillary(Medium Flow) 50µm |
EKG1275 |
|
Beckman–70cm Coated Capillary(Medium Flow) 50µm (Pack of 3) |
EKG1275-3 |
|
HP Format capillary fitted to Hewlett-Packard Cartridge
Description |
Cat. No. |
Price (£) |
|
HP – 25cm Coated Capillary (Medium Flow) 75µm |
EKG1325 |
|
HP 25cm Coated Capillary (Medium Flow) 7m (Pack of 3) |
EKG1325-3 |
|
HP 50cm Coated Capillary (Medium Flow) 7m |
EKG1350 |
|
HP – 50cm Coated Capillary (Medium Flow) 75µm (Pack of 3) |
EKG1350-3 |
|
HP – 75cm Coated Capillary (Medium Flow) 75µm |
EKG1375 |
|
HP – 75cm Coated Capillary (Medium Flow) 75µm (Pack of 3) |
EKG1375-3 |
|
HP – 25cm Coated Capillary (Medium Flow) 50µm |
EKH1325 |
|
HP 25cm Coated Capillary (Medium Flow) 5m (Pack of 3) |
EKH1325-3 |
|
HP 50cm Coated Capillary (Medium Flow) 5m |
EKH1350 |
|
HP – 50cm Coated Capillary (Medium Flow) 50µm (Pack of 3) |
EKH1350-3 |
|
HP – 25cm Coated Capillary (Medium Flow) 50µm |
EKH1375 |
|
HP – 25cm Coated Capillary (Medium Flow) 50µm (Pack of 3) |
EKH1375-3 |
|
TSP Format Capillary Fitted to TSP Cartridge
Description |
Cat. No. |
Price (£) |
|
TSP 25cm Coated Capillary (Medium Flow) 7m |
EKG1425 |
|
TSP 25cm Coated Capillary (Medium Flow) 7m (Pack of 3) |
EKG1425-3 |
|
TSP – 50cm Coated Capillary (Medium Flow) 75µm |
EKG1450 |
|
TSP – 50cm Coated Capillary (Medium Flow) 75µm (Pack of 3) |
EKG1450-3 |
|
TSP 75cm Coated Capillary (Medium Flow) 7m |
EKG1475 |
|
TSP – 75cm Coated Capillary (Medium Flow) 75µm (Pack of 3) |
EKG1475-3 |
|
TSP 25cm Coated Capillary (Medium Flow) 5m |
EKH1425 |
|
TSP 25cm Coated Capillary (Medium Flow) 5m (Pack of 3) |
EKH1425-3 |
|
TSP – 50cm Coated Capillary (Medium Flow) 50µm |
EKH1450 |
|
TSP – 50cm Coated Capillary (Medium Flow) 50µm (Pack of 3) |
EKH1450-3 |
|
TSP 75cm Coated Capillary (Medium Flow) 5m |
EKH1475 |
|
TSP – 75cm Coated Capillary (Medium Flow) 50µm (Pack of 3) |
EKH1475-3 |
|
Zero Flow Coated Capillaries
The open format capillaries are supplied precut to the required size with the UV window correctly positioned to fit the specific instrument manufacture’s cartridge system. The capillary ends are expertly cut to ensure precise sampling.
Description |
Cat. No. |
Price (£) |
|
25cm Coated Capillary (Zero Flow) 7m |
EKC1125 |
|
25cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1125-3 |
|
50cm Coated Capillary (Zero Flow) 75µm |
EKC1150 |
|
50cm Coated Capillary (Zero Flow) 7m (Pack of 3) |
EKC1150-3 |
|
75cm Coated Capillary (Zero Flow) 7m |
EKC1175 |
|
75cm Coated Capillary (Zero Flow) 7m (Pack of 3) |
EKC1175-3 |
|
25cm Coated Capillary (Zero Flow) 5m |
EKD1125 |
|
25cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1125-3 |
|
50cm Coated Capillary (Zero Flow) 50µm |
EKD1150 |
|
50cm Coated Capillary (Zero Flow) 5m (Pack of 3) |
EKD1150-3 |
|
75cm Coated Capillary (Zero Flow) 5m |
EKD1175 |
|
75cm Coated Capillary (Zero Flow) 5m (Pack of 3) |
EKD1175-3 |
|
Zero Flow Coated Capillaries – Cartridge Refit Service
Beckman format Total length is nominal length plus 7cm after detection window
Description |
Cat. No. |
Price (£) |
|
Beckman 25cm Coated Capillary (Zero Flow) 7m |
EKC1225 |
|
Beckman 25cm Coated Capillary (Zero Flow) 7m (Pack of 3) |
EKC1225-3 |
|
Beckman – 50cm Coated Capillary (Zero Flow) 75µm |
EKC1250 |
|
Beckman – 50cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1250-3 |
|
Beckman 70cm Coated Capillary (Zero Flow) 7m |
EKC1275 |
|
Beckman – 70cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1275-3 |
|
Beckman 25cm Coated Capillary (Zero Flow) 5m |
EKD1225 |
|
Beckman 25cm Coated Capillary (Zero Flow) 5m (Pack of 3) |
EKD1225-3 |
|
Beckman – 50cm Coated Capillary (Zero Flow) 50µm |
EKD1250 |
|
Beckman – 50cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1250-3 |
|
Beckman 70cm Coated Capillary (Zero Flow) 5m |
EKD1275 |
|
Beckman – 70cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1275-3 |
|
HP Format capillary fitted to Hewlett-Packard Cartridge
Description |
Cat. No. |
Price (£) |
|
HP – 25cm Coated Capillary (Zero Flow) 75µm |
EKC1325 |
|
HP 25cm Coated Capillary (Zero Flow) 7m (Pack of 3) |
EKC1325-3 |
|
HP 50cm Coated Capillary (Zero Flow) 7m |
EKC1350 |
|
HP – 50cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1350-3 |
|
HP – 75cm Coated Capillary (Zero Flow) 75µm |
EKC1375 |
|
HP – 75cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1375-3 |
|
HP – 25cm Coated Capillary (Zero Flow) 50µm |
EKD1325 |
|
HP 25cm Coated Capillary (Zero Flow) 5m (Pack of 3) |
EKD1325-3 |
|
HP 50cm Coated Capillary (Zero Flow) 5m |
EKD1350 |
|
HP – 50cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1350-3 |
|
HP – 25cm Coated Capillary (Zero Flow) 50µm |
EKD1375 |
|
HP – 25cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1375-3 |
|
TSP Format Capillary Fitted to TSP Cartridge
Description |
Cat. No. |
Price (£) |
|
TSP 25cm Coated Capillary (Zero Flow) 7m |
EKC1425 |
|
TSP 25cm Coated Capillary (Zero Flow) 7m (Pack of 3) |
EKC1425-3 |
|
TSP – 50cm Coated Capillary (Zero Flow) 75µm |
EKC1450 |
|
TSP – 50cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1450-3 |
|
TSP 75cm Coated Capillary (Zero Flow) 7m |
EKC1475 |
|
TSP – 75cm Coated Capillary (Zero Flow) 75µm (Pack of 3) |
EKC1475-3 |
|
TSP 25cm Coated Capillary (Zero Flow) 5m |
EKD1425 |
|
TSP 25cm Coated Capillary (Zero Flow) 5m (Pack of 3) |
EKD1425-3 |
|
TSP – 50cm Coated Capillary (Zero Flow) 50µm |
EKD1450 |
|
TSP – 50cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1450-3 |
|
TSP 75cm Coated Capillary (Zero Flow) 5m |
EKD1475 |
|
TSP – 75cm Coated Capillary (Zero Flow) 50µm (Pack of 3) |
EKD1475-3 |
|
 Anodic Flow Coated Capillaries (Positively Charged pH Independent)
From the earliest days of CE, it was realised that the poor peak shapes which were frequently observed with bases and biomolecules were mainly the result of interaction between the analyte and the capillary walls. Often protein separations were carried out at high pH well above their isoelectric points in order that the wall and the analyte were both negatively charged. Although effective for some separations, this approach effectively removes choice of pH as a separation variable.
In order to overcome this, Capital Analytical anodic flow capillaries have a permanent positive charge built into the polymer coating. This leads to a reversal of the normal direction of the electro-osmotic flow, i.e., towards the anode (anodic flow).This permits the separation of bases and basic proteins over a wider pH range (see application, right) Interaction with the wall leads to poor peak shape and low efficiency. With the anodic flow capillary sharp peaks are obtained for all compounds. Due to the presence of the fixed positive charges the EOF is in the opposite direction to that of bare silica.
The separation of the basic proteins shown in the example below would normally have to be carried out at alkaline pH values in order to try and ensure that the proteins and the capillary wall would be negatively charged. The example below illustrates the possibilities of using Capital Analytical anodic flow capillaries for the analysis of proteins at acid pH values. Not only does the use of anodic flow capillaries, as opposed to bare silica capillaries, result in a vastly improved peak shape, but the elution order of the components lysozyme, cytochrome C, myoglobinand ribonuclease A is completely reversed.
Ordering information
Anodic Flow Coated Capillaries The open format capillaries are supplied precut to the required size with the UV window correctly positioned to fit the specific instrument manufacture’s cartridge system. The capillary ends are expertly cut to ensure precise sampling.
Description |
Cat. No. |
Price (£) |
|
25cm Coated Capillary (Anodic Flow) 7m |
EKK1125 |
|
25cm Coated Capillary (Anodic Flow) 7m (Pack of 3) |
EKK1125-3 |
|
50cm Coated Capillary (Anodic Flow) 75µm |
EKK1150 |
|
50cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1150-3 |
|
75cm Coated Capillary (Anodic Flow) 7m |
EKK1175 |
|
75cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1175-3 |
|
25cm Coated Capillary (Anodic Flow) 5m |
EKL1125 |
|
25cm Coated Capillary (Anodic Flow) 5m (Pack of 3) |
EKL1125-3 |
|
50cm Coated Capillary (Anodic Flow) 50µm |
EKL1150 |
|
50cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1150-3 |
|
75cm Coated Capillary (Anodic Flow) 5m |
EKL1175 |
|
75cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1175-3 |
|
Anodic Flow Coated Capillaries – Cartridge Refit Service
Beckman format Total length is nominal length plus 7cm after detection window
Description |
Cat. No. |
Price (£) |
|
Beckman 20cm Coated Capillary (Anodic Flow) 7m |
EKK1225 |
|
Beckman 20cm Coated Capillary (Anodic Flow) 7m (Pack of 3) |
EKK1225-3 |
|
Beckman 50cm Coated Capillary (Anodic Flow) 75µm |
EKK1250 |
|
Beckman 50cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1250-3 |
|
Beckman 70cm Coated Capillary (Anodic Flow) 7m |
EKK1275 |
|
Beckman 70cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1275-3 |
|
Beckman 20cm Coated Capillary (Anodic Flow) 5m |
EKL1225 |
|
Beckman 20cm Coated Capillary (Anodic Flow) 5m (Pack of 3) |
EKL1225-3 |
|
Beckman 50cm Coated Capillary (Anodic Flow) 50µm |
EKL1250 |
|
Beckman 50cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1250-3 |
|
Beckman 70cm Coated Capillary (Anodic Flow) 5m |
EKL1275 |
|
Beckman 70cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1275-3 |
|
HP Format capillary fitted to Hewlett-Packard Cartridge
Description |
Cat. No. |
Price (£) |
|
HP – 20cm Coated Capillary (Anodic Flow) 75µm |
EKK1325 |
|
HP 20cm Coated Capillary (Anodic Flow) 7m (Pack of 3) |
EKK1325-3 |
|
HP 50cm Coated Capillary (Anodic Flow) 7m |
EKK1350 |
|
HP – 50cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1350-3 |
|
HP – 75cm Coated Capillary (Anodic Flow) 75µm |
EKK1375 |
|
HP – 75cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1375-3 |
|
HP – 20cm Coated Capillary (Anodic Flow) 50µm |
EKL1125 |
|
HP 20cm Coated Capillary (Anodic Flow) 5m (Pack of 3) |
EKL1125-3 |
|
HP 50cm Coated Capillary (Anodic Flow) 5m |
EKL1150 |
|
HP – 50cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1150-3 |
|
HP – 25cm Coated Capillary (Anodic Flow) 50µm |
EKL1175 |
|
HP – 25cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1175-3 |
|
TSP Format Capillary Fitted to TSP Cartridge
Description |
Cat. No. |
Price (£) |
|
TSP 20cm Coated Capillary (Anodic Flow) 7m |
EKK1425 |
|
TSP 20cm Coated Capillary (Anodic Flow) 7m (Pack of 3) |
EKK1425-3 |
|
TSP – 50cm Coated Capillary (Anodic Flow) 75µm |
EKK1450 |
|
TSP – 50cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1450-3 |
|
TSP 70cm Coated Capillary (Anodic Flow) 7m |
EKK1475 |
|
TSP – 70cm Coated Capillary (Anodic Flow) 75µm (Pack of 3) |
EKK1475-3 |
|
TSP 20cm Coated Capillary (Anodic Flow) 5m |
EKL1125 |
|
TSP 20cm Coated Capillary (Anodic Flow) 5m (Pack of 3) |
EKL1125-3 |
|
TSP – 50cm Coated Capillary (Anodic Flow) 50µm |
EKL1150 |
|
TSP – 50cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1150-3 |
|
TSP 70cm Coated Capillary (Anodic Flow) 5m |
EKL1175 |
|
TSP – 70cm Coated Capillary (Anodic Flow) 50µm (Pack of 3) |
EKL1175-3 |
|
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