Cyclodextrins and Buffer Reagents
 
 
 
 These macrocyclic carbohydrate compounds are widely used as buffer additives to obtain chiral separations in CE. However, they can also be used to adjust the selectivity in non chiral applications. There are three naturally occurring forms a, ß and ? which have 6, 7 and 8 glucose units respectively. The structure of ß-cyclodextrin is shown below. The hydroxyl units on the top of the cyclodextrin are chirally positioned and have different interactions with solute enantiomers when they are included into the cyclodextrin cavities. The hydroxyl groups can be chemically derivatised to give a range of neutral ad ionic derivatives which give different chiral separations and are more water soluble than the naturally occurring forms.

Structure of ß- cyclodextrin
Neutral cyclodextrin derivatives most frequently used in CE are:

   - dimethyl- cyclodextrin
   - trimethyl- cyclodextrin
   - hydroxypropyl- cyclodextrin

The most commonly used ionic forms are:

   - cyclodextrin sulphonic acid
   - Carboxymethyl- cyclodextrin
   - amino- cyclodextrin

The shape of cyclodextrins is often represented as a cone shaped cavity s shown below:

In order to obtain high efficiency separation it is necessary that the cyclodextrins used as CE additives are extremely pure. Capital Analytical technologies offers a wide range of both natural and derivatised cyclodextrins at CE grade purity. In addition to the cyclodextrins listed below, other derivatives are available on request. Please contact our sales office for details. Capital Analytical Cyclodextrins Capital Analytical has a full range of many types of cyclodextrins too numerous to list in full. If the cyclodextrin you require is not listed below, please contact our technical sales office.

The cyclodextrins form inclusion complexes with the different enatiomers which result in a chiral separation being obtained. A range of different sized and chemically modified cyclodextrins are used to obtain the required chiral separation.

Elphodextrin starter kit
Not sure which chiral Selector apply to your racemaic mixture? Elphodextrin starter Kit contains everything you need for rapid method development in Chiral CE separations. Seven cyclodextrins with different cavity size and cavity environment help you to follow the right track in your way toward complete separation. After a maximum 13 runs you achieve separation - many require no further optimisation.
Method development protocal and a flow chart are included, making the kit a complete tool for finding the suitable group type of cyclodextrin for the develeopment and optimisation of separation you need for your particular analyte.
(Ref. N. Roos et. al: J. Chromatography A 782(1997) 257-269)

The other Elphodex Kits contain wide variety of cyclodextrin derivatives for further optimisation of chiral separations. Choose Kit A, B or G with different cavity size or Acid and Phosphate Kit, when the use of ionic cyclodextrin is advantageous.

Contents

Part No.

Price £

  

Elphodextrin Starter Kit - 0.5g each of a, and gamma cyclodextrins, 0.5g dimethyl-ß- cyclodextrin, 0.5g 2-hydroxyproply-ß- cyclodextrin, 0.5g caboxymethyl- cyclodextrin, 0.5g monoamino- cyclodextrin

CYE7001

565.00
Qty

Elphodex A - , 2.5 g alpha-CD, 0.5 g methyl-alpha-CD, 0.5 g alpha-CD phosphate, 0.5 g permethyl-alpha-CD, 0.5 g (2-hydroxy)propyl-alpha-CD, 0.5 g carboxymethyl-alpha-CD, 0.5g alpa-CD phosphate

CYE70011

740.00
Qty

Elphodex B - 2.5g beta-CD, 0.5g permethyl-beta-CD, 0.5g methyl-beta-CD, 0.5g (2 hydroxy)Propyl-beta-CD, 0.5g carboxymethyl-beta-CD, 0.5g beta-CD phosphate

CYE70012

518.00
Qty

Elphodex G - 2.5g gamma-CD, 0.5g permethyl-gamma-CD, 0.5g (2 hydroxy)Propyl-gamma-CD, 0.5g carboxymethyl-gamma-CD, 0.5g gamma-CD phosphate

CYE70013

783.00
Qty

Elphodex Acid Kit - 0.5g Carboxymethyl-alpha-CD, 0.5g Carboxymethyl-beta-CD, 0.5g carboxymethyl-gamma-CD, 0.5g carboxyethyl-alpha-CD, 0.5g carboxyethyl-beta-CD, 0.5g carboxymethyl-gamma-CD

CYE70014

400.00
Qty

Elphodex Phosphate Kit - 0.5g alpha-CD Phosphate, low DS, 0.5g alpha-CD Phosphate, high DS, 0.5g beta-CD Phosphate, Low DS, 0.5g beta-CD phosphate, high DS, 0.5g gamma-CD phosphate, low DS, 0.5g gamma-CD phosphate, high DS

CYE70015

783.00
Qty

Type

Description

Qty

Cat No.

Price (£)

  

Natural Cyclodextrins

a - cyclodextrin 99%

5g

EKT522

322.00
Qty

Natural Cyclodextrins

a - cyclodextrin 99%

25g

EKT523

1288.00
Qty

Natural Cyclodextrins

b - cyclodextrin 99%

5g

EKT524

322.00
Qty

Natural Cyclodextrins

b - cyclodextrin 99%

25g

EKT525

1288.00
Qty

Natural Cyclodextrins

g - cyclodextrin 99%

1g

EKT526

138.00
Qty

Natural Cyclodextrins

g - cyclodextrin 99%

5g

EKT527

617.00
Qty

Modified Neutral Cyclodextrins

b - dimethyl cyclodextrin

5g

EKT528

65.00
Qty

Modified Neutral Cyclodextrins

b - dimethyl cyclodextrin

5g

EKT529

291.00
Qty

Modified Neutral Cyclodextrins

b - trimethyl cyclodextrin

5g

EKT530

291.00
Qty

Modified Neutral Cyclodextrins

b - trimethyl cyclodextrin

5g

EKT531

879.00
Qty

Modified Neutral Cyclodextrins

b - 2 - hydroxypropyl cyclodextrin

1g

EKT532

49.00
Qty

Modified Neutral Cyclodextrins

b - 2 - hydroxypropyl cyclodextrin

5g

EKT533

177.00
Qty

Ionic Modified Cyclodextrins

b-cyclodextrin phosphate

1g

EKT534

213.00
Qty

Ionic Modified Cyclodextrins

b-cyclodextrin phosphate

2.5g

EKT535

479.00
Qty

Ionic Modified Cyclodextrins

b - carboxy methyl cyclodextrin

1g

EKT536

43.00
Qty

Ionic Modified Cyclodextrins

b - carboxy methyl cyclodextrin

5g

EKT537

199.00
Qty

Ionic Modified Cyclodextrins

b - amino cyclodextrin

1 g

EKT538

578.00
Qty

Ionic Modified Cyclodextrins

b - amino cyclodextrin

5 g

EKT539

2776.00
Qty

Water Soluble Neutral Cyclodextrins

a-cyclodextrin ACD

1g

CY-E-1001

64.00
Qty

Water Soluble Neutral Cyclodextrins

b-cyclodextrin BCD

1g

CY-E-2001

64.00
Qty

Water Soluble Neutral Cyclodextrins

g-cyclodextrin GCD

1g

CY-E-3001

139.00
Qty

Water Soluble Neutral Cyclodextrins

Acetyl-a-cyclodextrin (DS ~7) AcACD

1g

CY-E-1002.1

139.00
Qty

Water Soluble Neutral Cyclodextrins

Acetyl-b-cyclodextrin (DS ~7) AcBCD

1g

CY-E-2002.1

96.00
Qty

Water Soluble Neutral Cyclodextrins

Acetyl-g-cyclodextrin (DS ~8) AcGCD

1g

CY-E-3002.1

138.00
Qty

Water Soluble Neutral Cyclodextrins

Acetyl-a-cyclodextrin(DS~14-16)AcACD-HDS

1g

CY-E-1002.2

138.00
Qty

Water Soluble Neutral Cyclodextrins

Acetyl-b-cyclodextrin (DS ~15) AcBCD-HDS

1g

CY-E-2002.2

104.00
Qty

Water Soluble Neutral Cyclodextrins

Acetyl-g-cyclodextrin (DS ~17) AcGCD-HDS

1g

CY-E-3002.2

138.00
Qty

Water Soluble Neutral Cyclodextrins

Hexakis(2,3,6-tri-O-methyl)-a-cyclodextrin TRIMEA

1g

CY-E-1003

369.00
Qty

Water Soluble Neutral Cyclodextrins

Heptakis(2,3,6-tri-O-methyl)-b-cyclodextrin TRIMEB

1g

CY-E-2003

231.00
Qty

Water Soluble Neutral Cyclodextrins

Octakis(2,3,6-tri-O-methyl)-g-cyclodextrin TRIMEG

1g

CY-E-3003

444.00
Qty

Water Soluble Neutral Cyclodextrins

Heptakis(2,6-di-O-methyl)-b-cyclodextrin DIMEB

1g

CY-E-2004.0

153.00
Qty

Water Soluble Neutral Cyclodextrins

Heptakis(2,6-di-O-methyl)-b-cyclodextrin, isomeric purity >80 % DIMEB/P

1g

CY-E-2004.2

234.00
Qty

Water Soluble Neutral Cyclodextrins

Heptakis(2,6-di-O-methyl)-b-cyclodextrin, isomeric purity >95 % DIMEB/P

1g

CY-E-2004.3

253.00
Qty

Water Soluble Neutral Cyclodextrins

Heptakis(2,6-di-O-methyl)-b-cyclodextrin, isomeric purity >98 % DIMEB/P

1g

CY-E-2004.4

299.00
Qty

Water Soluble Neutral Cyclodextrins

Randomly methylated a-cyclodextrin (DS ~11) RAMEA

1g

CY-E-1004.1

195.00
Qty

Water Soluble Neutral Cyclodextrins

Randomly methylated b-cyclodextrin (DS ~13) RAMEB

1g

CY-E-2004.1

67.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-a-cyclodextrin (DS ~3) HPACD

1g

CY-E-1005.1

138.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-b-cyclodextrin (DS ~3) HPBCD

1g

CY-E-2005.1

79.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-g-cyclodextrin (DS ~3) HPGCD

1g

CY-E-3005.1

203.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-a-cyclodextrin (DS ~4.8) HPACD

1g

CY-E-1005.2

138.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-b-cyclodextrin (DS ~4.6) HPBCD

1g

CY-E-2005.2

78.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-g-cyclodextrin (DS ~4.8) HPGCD

1g

CY-E-3005.2

253.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)propyl-b-cyclodextrin (DS ~6.3) HPBCD

1g

CY-E-2005.3

104.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)ethyl-a-cyclodextrin (DS ~4) HEACD

1g

CY-E-1028.1

289.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)ethyl-b-cyclodextrin (DS ~4) HEBCD

1g

CY-E-2028.1

165.00
Qty

Water Soluble Neutral Cyclodextrins

(2-hydroxy)ethyl-g-cyclodextrin (DS ~4) HEGCD

1g

CY-E-3028.1

289.00
Qty

Water Soluble Neutral Cyclodextrins

6-O-monomaltosyl-b-cyclodextrin, isomeric purity >98 % G2BCD/P

1g

CY-E-2042.0

406.00
Qty

Water Soluble Neutral Cyclodextrins

6-O-maltosyl-b-cyclodextrin (DS ~1.5) G2BCD

1g

CY-E-2042.1

277.00
Qty

Water Soluble Ionic Cyclodextrins

Carboxymethyl-a-cyclodextrin (DS ~3) CMACD

1g

CY-E-1006.0

104.00
Qty

Water Soluble Ionic Cyclodextrins

Carboxymethyl-b-cyclodextrin (DS ~3) CMBCD

1g

CY-E-2006.0

81.00
Qty

Water Soluble Ionic Cyclodextrins

Carboxymethyl-g-cyclodextrin (DS ~3) CMGCD

1g

CY-E-3006.0

272.00
Qty

Water Soluble Ionic Cyclodextrins

Succinyl-a-cyclodextrin (DS ~3.5) SuACD

1g

CY-E-1007.0

231.00
Qty

Water Soluble Ionic Cyclodextrins

Succinyl-b-cyclodextrin (DS ~3.5) SuBCD

1g

CY-E-2007.0

138.00
Qty

Water Soluble Ionic Cyclodextrins

Succinyl-g-cyclodextrin (DS ~3.5) SuGCD

1g

CY-E-3007.0

272.00
Qty

Water Soluble Ionic Cyclodextrins

Carboxyethyl-a-cyclodextrin (DS ~3) CEACD

1g

CY-E-1012.0

139.00
Qty

Water Soluble Ionic Cyclodextrins

Carboxyethyl-b-cyclodextrin (DS ~3) CEBCD

1g

CY-E-2012.0

110.00
Qty

Water Soluble Ionic Cyclodextrins

Carboxyethyl-g-cyclodextrin (DS ~3) CEGCD

1g

CY-E-3012.0

211.00
Qty

Water Soluble Ionic Cyclodextrins

a-cyclodextrin phosphate sodium salt (DS~2-6) PACD-LDS

1g

CY-E-1017.1

369.00
Qty

Water Soluble Ionic Cyclodextrins

b-cyclodextrin phosphate sodium salt (DS ~2-6) PBCD-LDS

1g

CY-E-2017.1

347.00
Qty

Water Soluble Ionic Cyclodextrins

g-cyclodextrin phosphate sodium salt (DS ~2-6) PGCD-LDS

1g

CY-E-3017.1

380.00
Qty

Water Soluble Ionic Cyclodextrins

a-cyclodextrin phosphate sodium salt (DS ~6-10) PACD-HDS

1g

CY-E-1017.2

278.00
Qty

Water Soluble Ionic Cyclodextrins

b-cyclodextrin phosphate sodium salt (DS ~6-10) PBCD-HDS

1g

CY-E-2017.2

278.00
Qty

Water Soluble Ionic Cyclodextrins

g-cyclodextrin phosphate sodium salt (DS ~6-10) PGCD-HDS

1g

CY-E-3017.2

308.00
Qty

Water Soluble Ionic Cyclodextrins

6-monodeoxy-6-monoamino-b-cyclodextrin hydrochloride MoAMBCD

1g

CY-E-2024

513.00
Qty

Water Soluble Ionic Cyclodextrins

Succinyl-(2-hydroxy)propyl-a-cyclodextrin SuHPACD

1g

CY-E-1033

381.00
Qty

Water Soluble Ionic Cyclodextrins

Succinyl-(2-hydroxy)propyl-b-cyclodextrin SuHPBCD

1g

CY-E-2033

326.00
Qty

Water Soluble Ionic Cyclodextrins

Succinyl-(2-hydroxy)propyl-g-cyclodextrin SuHPGCD

1g

CY-E-3033

381.00
Qty

Water Soluble Ionic Cyclodextrins

Sulfopropyl-a-cyclodextrin (DS ~2) SPACD

1g

CY-E-1040.0

326.00
Qty

Water Soluble Ionic Cyclodextrins

Sulfopropyl-b-cyclodextrin (DS ~2) SPBCD

1g

CY-E-2040.0

530.00
Qty

Water Soluble Ionic Cyclodextrins

Sulfopropyl-b-cyclodextrin (DS ~4) SPBCD4

1g

CY-E-2040.1

549.00
Qty

Water Soluble Ionic Cyclodextrins

Sulfopropyl-g-cyclodextrin (DS ~2) SPGCD

1g

CY-E-3040.0

530.00
Qty

Water Soluble Ionic Cyclodextrins

a-cyclodextrin sulfate sodium salt* SACD

1g

CY-E-1045

278.00
Qty

Water Soluble Ionic Cyclodextrins

b-cyclodextrin sulfate sodium salt* SBCD

1g

CY-E-2045

152.00
Qty

Water Soluble Ionic Cyclodextrins

g-cyclodextrin sulfate sodium salt* SGCD

1g

CY-E-3045

289.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Soluble a-cyclodextrin polymer CDPSA

1g

CY-E-1009

91.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Soluble b-cyclodextrin polymer CDPSB

1g

CY-E-2009

57.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Soluble g-cyclodextrin polymer CDPSG

1g

CY-E-3009

129.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Soluble anionic a-cyclodextrin polymer, carboxymethylated CDPSIA

1g

CY-E-1010

96.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Soluble anionic b-cyclodextrin polymer, carboxymethylated CDPSIB

1g

CY-E-2010

64.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Soluble anionic g-cyclodextrin polymer, carboxymethylated CDPSIG

1g

CY-E-3010

129.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Sulfated-a-cyclodextrin soluble polymer* SCDPSA

1g

CY-E-1046

484.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Sulfated b-cyclodextrin soluble polymer* SCDPSB

1g

CY-E-2046

404.00
Qty

Water Soluble Cyclodextrins - Neutral and ionic polymers

Sulfated-g-cyclodextrin soluble polymer* SCDPSG

1g

CY-E-3046

404.00
Qty

Water - Insoluble cyclodextrins

Hexakis(2,3,6-tri-O-acetyl)-a-cyclodextrin TRIAcACD

1g

CY-E-1002.0

193.00
Qty

Water - Insoluble cyclodextrins

Heptakis(2,3,6-tri-O-acetyl)-b-cyclodextrin TRIAcBCD

1g

CY-E-2002.0

112.00
Qty

Water - Insoluble cyclodextrins

Octakis(2,3,6-tri-O-acetyl)-g-cyclodextrin TRIAcGCD

1g

CY-E-3002.0

194.00
Qty

Water - Insoluble cyclodextrins

Heptakis(3-O-acetyl-2,6-di-O-methyl)-b-cyclodextrin AcDIMEB

1g

CY-E-2013

408.00
Qty

Water - Insoluble cyclodextrins

Heptakis(2,3,6-tri-O-benzoyl)-b-cyclodextrin TRIBenzBCD

1g

CY-E-2018.0

247.00
Qty

Water - Insoluble cyclodextrins

Ethyl-a-cyclodextrin (DS ~12) EtACD

1g

CY-E-1022.1

438.00
Qty

Water - Insoluble cyclodextrins

Ethyl-b-cyclodextrin (DS ~14) EtBCD

1g

CY-E-2022.1

372.00
Qty

Water - Insoluble cyclodextrins

Ethyl-g-cyclodextrin (DS ~16) EtGCD

1g

CY-E-3022.1

438.00
Qty

Water - Insoluble cyclodextrins

Heptakis(2,6-di-O-ethyl)-b-cyclodextrin DIEtBCD

1g

CY-E-2022.2

760.00
Qty

Water - Insoluble cyclodextrins

Heptakis(2,3,6-tri-O-ethyl)-b-cyclodextrin TRIEtBCD

1g

CY-E-2023

991.00
Qty

Water - Insoluble cyclodextrins

Heptakis(2,6-di-O-ethyl)-b-cyclodextrin DIEtBCD

1g

CY-E-2022.2

691.00
Qty

Water - Insoluble cyclodextrins

Heptakis(2,3,6-tri-O-ethyl)-b-cyclodextrin TRIEtBCD

1g

CY-E-2023

901.00
Qty

Type

Description

Qty

Cat No.

Price (£)

  
This reagent is widely used to rinse uncoated capillaries between injections in order to remove any adsorbed contaminants, which may otherwise cause variations in EOF, from the fused silica surface. The reagent is also used in order to reactivate uncoated fused silica capillaries before their initial use.

Buffer Preparation Reagent

0.1M Sodium Hydroxide

100ml

EKT510

6.00
Qty

Buffer Preparation Reagent

0.1M Sodium Hydroxide

250ml

EKT511

14.00
Qty
This solution is generally added to high pH (pH7-10) buffers to form the micelles necessary for Micellar Electrokinetic Capillary Chromatography (MECC). The CE based separation of electrically neutral compounds is generally achieved using this technique.

Buffer Preparation Reagent

200mM Sodium Dodecyl Sulphate (SDS)

100ml

EKT512

20.00
Qty

Buffer Preparation Reagent

200mM Sodium Dodecyl Sulphate (SDS)

250ml

EKT513

34.00
Qty
This is a standard buffer for the separation of basic compounds. It has, for example been used for the simultaneous resolution of 17 basic drugs. For the resolution of basic chiral compounds, such as pharmaceuticals and agrochemicals, cyclodextrins can be added to this buffer.

Buffer Preparation Reagent

100mM NaH2PO4 Phosphate Buffer adjusted to pH 2.5

100ml

EKT514

20.00
Qty

Buffer Preparation Reagent

100mM NaH2PO4 Phosphate Buffer adjusted to pH 2.5

250ml

EKT515

34.00
Qty
This is a useful alternative to pH2.5 phosphate buffer for the separation of basic compounds. Compared with the standard phosphate buffer it can often give improved peak shape and resolution. Highly efficient chiral separation of basic compounds can be achieved by the addition of cyclodextrins to this buffer.

Buffer Preparation Reagent

100mM Triethanolamine-Phosphoric Acid adjusted to pH 2.5

100ml

EKT516

20.00
Qty

Buffer Preparation Reagent

100mM Triethanolamine-Phosphoric Acid adjusted to pH 2.5

250ml

EKT517

34.00
Qty
This medium pH buffer is useful for the separation of a wide range of acidic compounds including agrochemicals, drugs and vitamins. It can be used in conjunction wit the 100mM SDS solution (EKT513) to produce a medium pH micellar buffer.

Buffer Preparation Reagent

100mM Phosphate Buffer adjusted to pH7 (50mm NaH2PO4 /50mm NaH2PO4)

100ml

EKT518

20.00
Qty

Buffer Preparation Reagent

100mM Phosphate Buffer adjusted to pH7 (50mm NaH2PO4 /50mm NaH2PO4)

250ml

EKT519

34.00
Qty
This solution has a natural pH of 9.4 and is useful for the separation of a wide range of acidic compounds. It generates a higher EOF than the pH7 phosphate buffer (cat. no.). Mixed with the SDS solution (cat. no.). An additional feature of the borate buffer is the ability of the borate ions to chelate with various diol compounds leading to a modified or enhanced selectivity.

Buffer Preparation Reagent

100mM Borax (100mM Sodium Tetraborate)

100ml

EKT520

20.00
Qty

Buffer Preparation Reagent

100mM Borax (100mM Sodium Tetraborate)

250ml

EKT521

34.00
Qty
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